A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene.
Identifieur interne : 001A05 ( Main/Exploration ); précédent : 001A04; suivant : 001A06A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene.
Auteurs : Qiong Zhao [République populaire de Chine] ; Jiayi Zhu ; Weijuan Zhu ; Xunliang Li ; Ye Tao ; Xiaonan Lv ; Xue Wang ; Jiechao Yin ; Cheng He ; Xiaofeng RenSource :
- Monoclonal antibodies in immunodiagnosis and immunotherapy [ 2167-9436 ] ; 2013.
Descripteurs français
- KwdFr :
- Analyse de variance, Animaux, Anticorps monoclonaux (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Hybridomes (immunologie), Plasmides (génétique), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Souris, Souris de lignée BALB C, Spécificité des anticorps, Technique d'immunofluorescence indirecte, Test ELISA, Virus de la gastroentérite transmissible (immunologie).
- MESH :
- génétique : Glycoprotéines membranaires, Plasmides, Protéines de l'enveloppe virale.
- immunologie : Anticorps monoclonaux, Glycoprotéines membranaires, Hybridomes, Protéines de l'enveloppe virale, Virus de la gastroentérite transmissible.
- Analyse de variance, Animaux, Glycoprotéine de spicule des coronavirus, Souris, Souris de lignée BALB C, Spécificité des anticorps, Technique d'immunofluorescence indirecte, Test ELISA.
English descriptors
- KwdEn :
- Analysis of Variance, Animals, Antibodies, Monoclonal (immunology), Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Indirect, Hybridomas (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Plasmids (genetics), Spike Glycoprotein, Coronavirus, Transmissible gastroenteritis virus (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal, Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Plasmids.
- immunology : Hybridomas, Transmissible gastroenteritis virus.
- Analysis of Variance, Animals, Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Indirect, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus.
Abstract
Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the hybridoma technique. The generated MAb (1H4) was identified by ELISA. Immunofluorescence assays showed that MAb 1H4 was able to detect infection of cells with TGEV. The MAb 1H4 distinguished TGEV from other control viruses. Additionally, although the type of MAb 1H4 was IgM, it could reduce cell infection by TGEV in a dose-dependent manner.
DOI: 10.1089/mab.2012.0067
PubMed: 23600507
Affiliations:
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Le document en format XML
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gene.</title><author><name sortKey="Zhao, Qiong" sort="Zhao, Qiong" uniqKey="Zhao Q" first="Qiong" last="Zhao">Qiong Zhao</name><affiliation wicri:level="1"><nlm:affiliation>College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Veterinary Medicine, Northeast Agricultural University, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Zhu, Jiayi" sort="Zhu, Jiayi" uniqKey="Zhu J" first="Jiayi" last="Zhu">Jiayi Zhu</name></author><author><name sortKey="Zhu, Weijuan" sort="Zhu, Weijuan" uniqKey="Zhu W" first="Weijuan" last="Zhu">Weijuan Zhu</name></author><author><name sortKey="Li, Xunliang" sort="Li, Xunliang" uniqKey="Li X" first="Xunliang" last="Li">Xunliang Li</name></author><author><name sortKey="Tao, Ye" sort="Tao, Ye" uniqKey="Tao Y" first="Ye" last="Tao">Ye Tao</name></author><author><name sortKey="Lv, Xiaonan" sort="Lv, Xiaonan" uniqKey="Lv X" first="Xiaonan" last="Lv">Xiaonan Lv</name></author><author><name sortKey="Wang, Xue" sort="Wang, Xue" uniqKey="Wang X" first="Xue" last="Wang">Xue Wang</name></author><author><name sortKey="Yin, Jiechao" sort="Yin, Jiechao" uniqKey="Yin J" first="Jiechao" last="Yin">Jiechao Yin</name></author><author><name sortKey="He, Cheng" sort="He, Cheng" uniqKey="He C" first="Cheng" last="He">Cheng He</name></author><author><name sortKey="Ren, Xiaofeng" sort="Ren, Xiaofeng" uniqKey="Ren X" first="Xiaofeng" last="Ren">Xiaofeng Ren</name></author></analytic><series><title level="j">Monoclonal antibodies in immunodiagnosis and immunotherapy</title><idno type="eISSN">2167-9436</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Antibody Specificity</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fluorescent Antibody Technique, Indirect</term><term>Hybridomas (immunology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Plasmids (genetics)</term><term>Spike Glycoprotein, Coronavirus</term><term>Transmissible gastroenteritis virus (immunology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de variance</term><term>Animaux</term><term>Anticorps monoclonaux (immunologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Hybridomes (immunologie)</term><term>Plasmides (génétique)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps</term><term>Technique d'immunofluorescence indirecte</term><term>Test ELISA</term><term>Virus de la gastroentérite transmissible (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Plasmides</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Glycoprotéines membranaires</term><term>Hybridomes</term><term>Protéines de l'enveloppe virale</term><term>Virus de la gastroentérite transmissible</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hybridomas</term><term>Transmissible gastroenteritis virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Antibody Specificity</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fluorescent Antibody Technique, Indirect</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de variance</term><term>Animaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps</term><term>Technique d'immunofluorescence indirecte</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the hybridoma technique. The generated MAb (1H4) was identified by ELISA. Immunofluorescence assays showed that MAb 1H4 was able to detect infection of cells with TGEV. The MAb 1H4 distinguished TGEV from other control viruses. Additionally, although the type of MAb 1H4 was IgM, it could reduce cell infection by TGEV in a dose-dependent manner.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="He, Cheng" sort="He, Cheng" uniqKey="He C" first="Cheng" last="He">Cheng He</name><name sortKey="Li, Xunliang" sort="Li, Xunliang" uniqKey="Li X" first="Xunliang" last="Li">Xunliang Li</name><name sortKey="Lv, Xiaonan" sort="Lv, Xiaonan" uniqKey="Lv X" first="Xiaonan" last="Lv">Xiaonan Lv</name><name sortKey="Ren, Xiaofeng" sort="Ren, Xiaofeng" uniqKey="Ren X" first="Xiaofeng" last="Ren">Xiaofeng Ren</name><name sortKey="Tao, Ye" sort="Tao, Ye" uniqKey="Tao Y" first="Ye" last="Tao">Ye Tao</name><name sortKey="Wang, Xue" sort="Wang, Xue" uniqKey="Wang X" first="Xue" last="Wang">Xue Wang</name><name sortKey="Yin, Jiechao" sort="Yin, Jiechao" uniqKey="Yin J" first="Jiechao" last="Yin">Jiechao Yin</name><name sortKey="Zhu, Jiayi" sort="Zhu, Jiayi" uniqKey="Zhu J" first="Jiayi" last="Zhu">Jiayi Zhu</name><name sortKey="Zhu, Weijuan" sort="Zhu, Weijuan" uniqKey="Zhu W" first="Weijuan" last="Zhu">Weijuan Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Qiong" sort="Zhao, Qiong" uniqKey="Zhao Q" first="Qiong" last="Zhao">Qiong Zhao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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